T Cell Activation & Proliferation Assay

The interaction between activated immune cells themselves with antigen-presenting cells is an important part to regulate the immune response, since during the immune response the activated T lymphocytes undergo rapid expansion to target and attack the tumor. T cell activation can be measured in multiple ways, including proliferation, up-regulation of activation markers (e.g., IL2RA/CD25), and production of effector cytokines such as IFN-γ and TNF-α.

ProMab offers T cell activation and proliferation assays to validate the anti-tumor activity of immuno-oncology (IO) drugs. We can provide commercial kits to conduct the assay or can also customize the assay for research and development projects.

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ProMab Biotechnologies Custom T Cell Activation and Proliferation

T cell activation is initiated by the engagement of TCR and CD3 complex, and the subsequent engagement of co-stimulatory molecules, such as CD28 receptor. After co-stimulation of these two signals, a series of intracellular activations happen, including the cytokine release and T cell proliferation in order to fight infection or disease.

ProMab has developed a systematic approach to T cell activation and proliferation assays for IO products discovery. The nuclear factor of activated T cells (NFAT), IL-2 or other kinds of factors will be detected by bioluminescent methods or ELISA kits. Meanwhile, the T cell activation and proliferation process can also be evaluated via cell counting kit-8 (CCK-8), MTT, CFSE as well as flow cytometric analysis, etc.

The process of generating and expanding CAR-T cells has been improved with an optimized CAR-T expansion media (cat.# PM-CAR2000).

Our T cell activation and expansion platform mimics what occurs in nature, but with greater efficiency and control.

Figure 1. Scheme of T cell activation in nature (top) and in ProMab's platform (bottom)

Figure 2. Representative growth profile of transduced cells.

Figure 3. Morphology of transduced cells (Day 10).

2~3x10^9 cells can be produced using ProMab's CAR-T medium (#PM-CAR2000) and CD3/CD28 T cell activation beads (#PM-CAR2002) (Figure 1). The growth of the cells is monitored by counting (Figure 2) and microscopy (Figure 3).