Stable Cell Line Development
ProMab Biotechnologies has 2 decades of experience generating highly specific and potent monoclonal antibodies. We have generated thousands of hybridoma cell lines for our catalog and custom projects for clients from academic institutions and pharmaceutical companies. We have expanded our offerings to provide a proprietary lentiviral and transfection-based stable cell line development service for research and biopharma applications.
Stably-transfected cell lines are used for many drug discovery assays such as screening and validation. Compared to traditional antibody production methods, utilizing a stable cell line overcomes many production issues and inconsistencies that come with hybridoma production or standard transient expression. For cost-effective large-scale production, a stable cell line is the way to express your gene of interest. A stable cell line allows for persistent and reproducible expression of your gene of interest over many passages. Our capabilities can utilize most mammalian cell lines including HEK293, CHO, and even CHO-K1.
ProMab offers a complete package for stable cell line development utilizing our proprietary expression vector or we can start from yours. The time-consuming and strenuous process will be handled by our team of experts to develop the stable cell lines you need for protein production and cell-based assays. Further assay development and characterization can also be done at our R&D facility, including but not limited to flow cytometry, immunohistochemistry, western blot analysis, SPR, and many more.
Included in custom stable cell line engineering service:
- Plasmid Design & Synthesis
- Transduction or Transfection
- Selection from pool of cells
- Clonal screening and selection
- Cell line characterization
- Expansion and downstream evaluation
Antibody & Protein Stable Cell Line Development
| Stable Cell Line Production | Cost |
|---|---|
| DNA reconstruction of recombinant antibody in lentivirus or patented plasmid vector. Transduction or transfection into a high expressing system of a human cell line. Single-cell screening and clone selection for the highest-producing clone. Production: Protein expression and purification from 500 mL culture. |
Inquire |
| Master and Working Cell Banking Services | Inquire |
Other Custom Services:
Mouse monoclonal antibodies
Human monoclonal antibodies
Hybridoma Sequencing