Mouse Monoclonal Antibodies

The first step to generate the desired antibodies involves immunizing Balc/c mice with a specific antigen. If you require a custom monoclonal antibody production, our team at ProMab can help you design the peptide sequence for your project. While immunizing mice produces high-affinity and specific antibodies, it is possible for certain antigens and mice to have a stronger immune response than others. To receive a mature immune response, 5 injections of antigen into mice are typically needed; however, it is possible that more or less immunization boosts are required which would increase or decrease the timeline by another 2 to 4 weeks to get the desired titer. Generally, immunization will take approximately 10 weeks. 

Following sacrifice, the extracted spleen cells will be fused with compatible myeloma cell lines (Sp 2/0 cells) to generate the hybridoma cells for selection via ELISA. The presence of the desired antibodies is identified using ELISA but additional services including western blot and flow cytometry are available upon request. 

The supernatant from 10 hybridoma clones (or more if selected) will be selected based on ELISA performance and sent to the client for in-house evaluation. The client can choose two clones (or more if selected) which we will further subclone and assay to ensure monoclonality and stability. The Custom Mouse Monoclonal Antibody Package starts at $6,995.

Phase III

Upon confirmation and selection of the desired hybridoma clones, the chosen hybridoma cells will be subcloned to ensure stability and monoclonal characteristic of the cell. The limiting-dilution method will isolate individual hybridomas from the mixed population of cells. The isolated cells will then be expanded and cryopreserved for shipment. If you have any questions about our mouse monoclonal antibody production service, or if you are looking to request a quote, please click below: 

 

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