ZAP70 Primary Antibody
Item Information
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Size
Price
Description
ZAP70 (zeta-chain associated protein kinase), a 70 kDa member of the SYK tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. ZAP70 controls TCR(T-cell antigen receptor)-linked signal transduction pathways. Its key role in thymocytes development and mature T lymphocytes activation has been illustrated by the characterization of several human immunodeficiencies presenting with mutations in the ZAP70 gene. ZAP70 is also expressed in several types of B-cell neoplasm and is easily detected by immunohistochemistry, providing a useful prognostic marker in patients with chronic lymphocytic leukaemia.
Product Overview
Entrez GenelD
7535
Aliases
SRK; STD; TZK
Clone#
3D2A4
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human ZAP70 expressed in E. Coli.
Formulation
Ascitic fluid containing 0.03% sodium azide.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ELISA
1/10000
References
1. Sigal Gelkop , Gerrald D. Gish, Yael Babichev J Immunol. 2005 Dec 15;175(12):8123-32.
2. Joaquim Carreras , Neus Villamor ,Lluis Colomo J Pathol. 2005 Mar;205(4):507-13.
3. Claire Hivroz Med Sci (Paris). 2005 Feb;21(2):150-5.
2. Joaquim Carreras , Neus Villamor ,Lluis Colomo J Pathol. 2005 Mar;205(4):507-13.
3. Claire Hivroz Med Sci (Paris). 2005 Feb;21(2):150-5.
Product Image
Western Blot
Figure 1: Western blot analysis using ZAP70 mouse mAb against Jurkat cell lysate (1).
Immunohistochemical analysis
Figure 2: Immunohistochemical analysis of paraffin-embedded human lymph tumor, showing cytoplasmic localization using ZAP70 mouse mAb with DAB staining
For Research Use Only. Not for use in diagnostic procedures.