PTPN14 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an N-terminal noncatalytic domain similar to that of band 4.1 superfamily cytoskeleton-associated proteins, which suggested the membrane or cytoskeleton localization of this protein. It appears to regulate lymphatic development in mammals, and a loss of function mutation has been found in a kindred with a lymphedema-choanal atresia.
Product Overview
Entrez GenelD
5784
Aliases
PEZ; PTP36
Clone#
2B3H7
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PTPN14 (AA: 896-1169) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Oncogene. 2013 Apr 18;32(16):2087-95.
2.PLoS One. 2013 Apr 16;8(4):e61916.
2.PLoS One. 2013 Apr 16;8(4):e61916.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using PTPN14 mAb against HEK293 (1) and PTPN14 (AA: 896-1169)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3:Western blot analysis using PTPN14 mAb against human PTPN14 (AA: 896-1169) recombinant protein. (Expected MW is 57.5 kDa)
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using PTPN14 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 5:Flow cytometric analysis of Hela cells using PTPN14 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using PTPN14 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PTPN14 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.