PRL Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein.

Product Overview

Entrez GenelD

5617

Clone#

2H10B2

Host / Isotype

Mouse / IgG2b

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human PRL (AA: 65-173) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. J Neuroendocrinol. 2012 Jul;24(7):977-90.
2. Mol Cell Endocrinol. 2012 Jun 5;356(1-2):80-7.

Product Image

Western Blot

Figure 1: Western blot analysis using PRL mAb against human PRL (AA: 65-173) recombinant protein. (Expected MW is 38.5 kDa)

Western Blot

Figure 2: Western blot analysis using PRL mAb against HEK293 (1) and PRL (AA: 65-173)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of Hela cells using PRL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunofluorescence analysis

Figure 4: Immunofluorescence analysis of MCF-7 cells using PRL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric