Mouse Monoclonal Antibody to PRF1
Item Information
Catalog #
Size
Price
Description
This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood.
Product Overview
Entrez GenelD
5551
Aliases
P1; PFP; HPLH2
Clone#
8A8C10
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human PRF1 (AA: extra 148-372) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.BMC Pediatr. 2017 Mar 3;17(1):65. 2.Pan Afr Med J. 2020 Aug 27;36:354.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/1ee954779d8ba23466987f5865c0502103b471b813431a52d56f15cc685a1e0e.jpg?v=1666705797)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using PRF1 mAb against human PRF1 (AA: extra 148-372) recombinant protein. (Expected MW is 29.7 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/5baa1e124a135e317dd86b3cf669ebf1b1682089153bc001d78d2aee8d049337.jpg?v=1666705797)
Figure 2:Western blot analysis using PRF1 mAb against human PRF1 (AA: extra 148-372) recombinant protein. (Expected MW is 29.7 kDa)
Western Blot
![Figure 3:Western blot analysis using PRF1 mAb against HEK293-6e (1) and PRF1 (AA: extra 148-372)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/5de15c220be14797ca14c6ac71f81fdb43d60e384563e6d507e4894ef998953a.jpg?v=1666705797)
Figure 3:Western blot analysis using PRF1 mAb against HEK293-6e (1) and PRF1 (AA: extra 148-372)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
![Figure 4:Western blot analysis using PRF1 mouse mAb against mouse liver (1) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e85ad35d564c3d1e5c1c4a4d6eb4f0775ee89cfc1be9fc1559090d66c7a52529.jpg?v=1666705797)
Figure 4:Western blot analysis using PRF1 mouse mAb against mouse liver (1) cell lysate.
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of Jurkat cells using PRF1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/4c83ed4fee109fadb8dd0fa56519a625ca726ead385e6a0c082d27bda50bc048.jpg?v=1666705797)
Figure 5:Flow cytometric analysis of Jurkat cells using PRF1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PRF1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/1f41d74e8b3ed2f383df5ee4223518960c04206af688200276cf9a5839091f57.jpg?v=1666705797)
Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PRF1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.