POU5F1
Item Information
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Price
Description
This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12.
Product Overview
Entrez GenelD
5460
Aliases
OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
Clone#
2D9H4
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human POU5F1 (AA: 136-360) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/50 - 1/200
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Biomark Med. 2020 Oct;14(15):1473-1484.
2.Cancer Med. 2020 Dec;9(23):8782-8800.
2.Cancer Med. 2020 Dec;9(23):8782-8800.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using POU5F1 mAb against human POU5F1 (AA: 136-360) recombinant protein. (Expected MW is 65.2 kDa)
Western Blot
Figure 3:Western blot analysis using POU5F1 mAb against HEK293-6e (1) and POU5F1 (AA: 136-360）-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunohistochemical analysis
Figure 4:Immunofluorescence analysis of Hela cells using POU5F1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 5:Flow cytometric analysis of K562 cells using POU5F1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.