PMS2 Primary Antibody

Item Information

Catalog #

Size

Price

Description

This gene is one of the PMS2 gene family members found in clusters on chromosome 7. The product of this gene is involved in DNA mismatch repair. It forms a heterodimer with MLH1 and this complex interacts with other complexes bound to mismatched bases. Mutations in this gene are associated with hereditary nonpolyposis colorectal cancer, Turcot syndrome, and are a cause of supratentorial primitive neuroectodermal tumors. Alternatively spliced transcript variants have been observed for this gene.

Product Overview

Entrez GenelD

5395

Aliases

PMSL2; HNPCC4; PMS2CL

Clone#

1E9D11

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human PMS2 (AA: 748-851) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.J Med Genet. 2013 Aug;50(8):552-63.
2.Hum Mutat. 2010 May;31(5):552-60.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);

Western Blot

Figure 2:Western blot analysis using PMS2 mAb against human PMS2 (AA: 748-851) recombinant protein. (Expected MW is 37.7 kDa)

Western Blot

Figure 3:Western blot analysis using PMS2 mAb against HEK293 (1) and PMS2 (AA: 748-851)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 4:Immunofluorescence analysis of HeLa cells using PMS2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of MCF-7 cells using PMS2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric