PIK3R4 Primary Antibody
Item Information
Catalog #
Size
Price
Description
PI 3-Kinases (phosphoinositide 3-kinases, PI 3-Ks) are a family of lipid kinases capable of phosphorylating the 3'OH of the inositol ring of phosphoinositides. They are responsible for coordinating a diverse range of cell functions including proliferation and survival.
Product Overview
Entrez GenelD
30849
Aliases
p150; VPS15
Clone#
5H3A11
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human PIK3R4 (AA: 772-906) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4?; -20? for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Nat Commun. 2016 Nov 24;7:13586. 2.Leuk Res. 2015 Jun;39(6):555-60.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using PIK3R4 mAb against human PIK3R4 (AA: 772-906) recombinant protein. (Expected MW is 40.8 kDa)
Western Blot
Figure 3:Western blot analysis using PIK3R4 mAb against HEK293 (1) and PIK3R4 (AA: 772-906)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of Hela cells using PIK3R4 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow Cytometric
Figure 5:Flow cytometric analysis of Hela cells using PIK3R4 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.