Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using PDHA1 mAb against human PDHA1 (AA: 241-390) recombinant protein. (Expected MW is 37.5 kDa)
Western Blot
Figure 3:Western blot analysis using PDHA1 mAb against HEK293-6e (1) and PDHA1 (AA: 241-390)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using PDHA1 mouse mAb against HepG2 (1), HEK293 (2), HL-60 (3), SK-OV-3 (4), PC-3 (5), PANC-1 (6), NRK (7), C2C12 (8), C6 (9) and PC-12 (10) cell lysate.
Western Blot
Figure 5: Immunoprecipitation using PDHA1 mouse mAb(dilution: 1/250) against HL-60, C2C12, and NRK cell lysate. Western blot analysis using PDHA1 mouse mAb, anti-mouse IgG was used as secondary antibody. Lane 1: cell lysate, Lane 2: Normal Mouse IgG instead of PDHA1 mouse mAb IP in cell lysate, Lane 3: PDHA1 mouse mAb IP in cell lysate.
Immunohistochemical analysis
Figure 6:Immunofluorescence analysis of Hela cells using PDHA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunohistochemical analysis
Figure 7:Immunofluorescence analysis of NIH/3T3 cells using PDHA1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 8:Flow cytometric analysis of Hela cells using PDHA1 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
Figure 9:Flow cytometric analysis of Jurkat cells using PDHA1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 10:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using PDHA1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 11:Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PDHA1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 12:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using PDHA1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 13:Immunohistochemical analysis of paraffin-embedded mouse kidney tissues using PDHA1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 14:Immunohistochemical analysis of paraffin-embedded Rat kidney tissues using PDHA1 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 15:Immunohistochemical analysis of paraffin-embedded Rabbit kidney tissues using PDHA1 mouse mAb with DAB staining.
Western Blot
Figure 15:Western blot analysis using *** mouse mAb against *** (1), *** (2), and *** (*) cell lysate.