PD1 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases.
Product Overview
Entrez GenelD
5133
Aliases
PDCD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
Clone#
3E12A10
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human PD1 (AA: extra 21-170) expressed in HEK293 cells.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.PLoS One. 2016 Jul 26;11(7):e0159383.
2.Tumour Biol. 2016 Jun;37(6):7507-14.
2.Tumour Biol. 2016 Jun;37(6):7507-14.
Product Image
Elisa
![Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31420ea.jpg?v=1666702844)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using PD1 mAb against human PD1 (AA: extra 21-170) recombinant protein. (Expected MW is 46.6 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31420rwb.jpg?v=1666702844)
Figure 2:Western blot analysis using PD1 mAb against human PD1 (AA: extra 21-170) recombinant protein. (Expected MW is 46.6 kDa)
Immunofluorescence analysis
![Figure 3:Immunofluorescence analysis of Hela cells using PD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31420icc.jpg?v=1666702844)
Figure 3:Immunofluorescence analysis of Hela cells using PD1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
![Figure 4:Flow cytometric analysis of HL-60 cells using PD1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31420fcm.jpg?v=1666702844)
Figure 4:Flow cytometric analysis of HL-60 cells using PD1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.