Mouse Monoclonal Antibody to NEFL
Item Information
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Size
Price
Description
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]
Product Overview
Entrez GenelD
4747
Aliases
NFL; NF-L; NF68; CMT1F; CMT2E; CMTDIG; PPP1R110
Clone#
2F8B3
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human NEFL (AA: 1-200) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1，Brain. 2020 Jan 1;143(1):47-54. 2，Lancet Neurol. 2019 Dec;18(12):1103-1111.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using NEFL mAb against human NEFL (AA: 1-200) recombinant protein. (Expected MW is 26.9 kDa)
Western Blot
Figure 3:Western blot analysis using NEFL mAb against HEK293-6e (1) and NEFL (AA: 1-200)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Jurkat cells using NEFL mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 5:Flow cytometric analysis of SK-N-SH cells using NEFL mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded brain tissues using NEFL mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using NEFL mouse mAb with DAB staining.
Western Blot
Figure 8:Western blot analysis using NEFL mouse mAb against HEK293 (1) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.