Mouse Monoclonal Antibody to NEDD9
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a member of the CRK-associated substrates family. Members of this family are adhesion docking molecules that mediate protein-protein interactions for signal transduction pathways. This protein is a focal adhesion protein that acts as a scaffold to regulate signaling complexes important in cell attachment, migration and invasion as well as apoptosis and the cell cycle. This protein has also been reported to have a role in cancer metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2012]
Product Overview
Entrez GenelD
4739
Aliases
CAS2; CASL; HEF1; CAS-L; CASS2
Clone#
3F7A9
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human NEDD9 (AA: 82-398) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Biomolecules.2018 Dec 10;8(4):169.2.Biochem Biophys Res Commun.2019 Jan 29;509(1):201-208.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/a3ad5f7677354782d3f1066554b0c82a89d2276e6a6179c10a46d67189ee1c76.jpg?v=1666706073)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using NEDD9 mAb against human NEDD9 (AA: 82-398) recombinant protein. (Expected MW is 37.3 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/bbd48a0b085a6345d4926ef0a8b6231a45bca51513bb3ec22012915b376749cf.jpg?v=1666706073)
Figure 2:Western blot analysis using NEDD9 mAb against human NEDD9 (AA: 82-398) recombinant protein. (Expected MW is 37.3 kDa)
Western Blot
![Figure 4:Western blot analysis using NEDD9 mouse mAb against MCF-7 (1), Hela (2), C2C12 (3),and Hek293 (4) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/6d6e02e149d9db7cd43db2c99b182df7854433d181b89810b43e6035e4db14dd.jpg?v=1666706073)
Figure 4:Western blot analysis using NEDD9 mouse mAb against MCF-7 (1), Hela (2), C2C12 (3),and Hek293 (4) cell lysate.
Immunofluorescence analysis
![Figure 5:Immunofluorescence analysis of Hela cells using NEDD9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/f61591c5b6ee10a6a5d5348a2c63d40336f4500a2a7e234a43072c16f149b2d5.jpg?v=1666706073)
Figure 5:Immunofluorescence analysis of Hela cells using NEDD9 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
![Figure 6:Flow cytometric analysis of HepG2 cells using NEDD9 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/fd0217021cc16a87bfa4d56de8bdc9c5ecba9bd14478b8b205930ce5e8deaf78.jpg?v=1666706073)
Figure 6:Flow cytometric analysis of HepG2 cells using NEDD9 mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 7:Flow cytometric analysis of Jurkat cells using NEDD9 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/7da44d0a0f8ffe939b8ca0e1c828804d98c8c336f358ec8f3d68f445a038a246.jpg?v=1666706073)
Figure 7:Flow cytometric analysis of Jurkat cells using NEDD9 mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 8:Flow cytometric analysis of K562 cells using NEDD9 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/f6e0e8e6bcf786315bdf1ba2ccb8f2a0100df6aa2f8868f672244f0700657312.jpg?v=1666706073)
Figure 8:Flow cytometric analysis of K562 cells using NEDD9 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.