MAPK10 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as integration points for multiple biochemical signals and are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is specifically expressed in a subset of neurons in the nervous system and is activated by threonine and tyrosine phosphorylation. Targeted deletion of this gene in mice suggests that it may have a role in stress-induced neuronal apoptosis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon.
Product Overview
Entrez GenelD
5602
Aliases
JNK3; JNK3A; PRKM10; SAPK1b; p493F12; p54bSAPK
Clone#
2B10H12
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human, Rat
Immunogen
Purified recombinant fragment of human MAPK10 (AA: 180-329) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1.J Diabetes Res. 2014;2014:814854.
2.Pathol Int. 2011 Jan;61(1):52-4.
2.Pathol Int. 2011 Jan;61(1):52-4.
Product Image
Elisa
![Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31038ea.jpg?v=1666701649)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using MAPK10 mAb against human MAPK10 (AA: 180-329) recombinant protein. (Expected MW is 43.1 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31038rwb.jpg?v=1666701649)
Figure 2:Western blot analysis using MAPK10 mAb against human MAPK10 (AA: 180-329) recombinant protein. (Expected MW is 43.1 kDa)
Western Blot
![Figure 3:Western blot analysis using MAPK10 mAb against HEK293 (1) and MAPK10 (AA: 180-329)-hIgGFc transfected HEK293 (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31038twb.jpg?v=1666701649)
Figure 3:Western blot analysis using MAPK10 mAb against HEK293 (1) and MAPK10 (AA: 180-329)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
![Figure 4:Western blot analysis using MAPK10 mouse mAb against HEK293 (1), Hela (2), SK-N-SH (3), MCF-7 (4), Jurkat (5), and C6 (6) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/31038lwb.jpg?v=1666701649)
Figure 4:Western blot analysis using MAPK10 mouse mAb against HEK293 (1), Hela (2), SK-N-SH (3), MCF-7 (4), Jurkat (5), and C6 (6) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.