Mouse Monoclonal Antibody to MAGEA4
Item Information
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Price
Description
This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. Several variants encoding the same protein have been found for this gene.
Product Overview
Entrez GenelD
4103
Aliases
CT1.4; MAGE4; MAGE4A; MAGE4B; MAGE-41; MAGE-X2
Clone#
1E4C4
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human MAGEA4 (AA: 1-225) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Cancer Res Ther. 2018 Jul-Sep;14(5):1059-1064. 2.Int J Oncol. 2018 Aug;53(2):713-724.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using MAGEA4 mAb against human MAGEA4 (AA: 1-225) recombinant protein. (Expected MW is 27.2 kDa)
Western Blot
Figure 3:Western blot analysis using MAGEA4 mAb against HEK293-6e (1) and MAGEA4 (AA: 1-225)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using MAGEA4 mouse mAb against Mouse liver (1), Rat liver (2) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of LNCAP cells using MAGEA4 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.