LSD1/AOF2 Primary Antibody

Item Information

Catalog #

Size

Price

Description

The amine oxidase domain 2 (AOF2) gene encodes a nuclear protein (LSD1, ~95kDa) containing a Swirm domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. LSD1 is a chromatin-modifying enzyme, which serve as a docking module for the stabilization of the associated corepressor complex (es) on chromatin.

Product Overview

Entrez GenelD

23028

Aliases

KDM1; LSD1; AOF2

Clone#

1B2E5

Species Reactivity

Human, Mouse, Monkey

Immunogen

Purified recombinant fragment of human LSD1 expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. Shi YJ, et.al Mol Cell. 2005 Sep 16;19(6):857-64.
2. Metzger E, et.al Nature. 2005 Sep 15;437(7057):436-9.

Product Image

Western Blot

Figure 1: Western blot analysis using LSD1 mouse mAb against COS (1), Hela (2), NIH/3T3 (3), A549 (4) and Jurkat (5) cell lysate.

Immunohistochemical analysis

Figure 2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma (left) and kidney carcinoma (right), showing nuclear localization using LSD1 mouse mAb with DAB staining.

For Research Use Only. Not for use in diagnostic procedures.