Mouse Monoclonal Antibody to KIT
This gene encodes a receptor tyrosine kinase. This gene was initially identified as a homolog of the feline sarcoma viral oncogene v-kit and is often referred to as proto-oncogene c-Kit. The canonical form of this glycosylated transmembrane protein has an N-terminal extracellular region with five immunoglobulin-like domains, a transmembrane region, and an intracellular tyrosine kinase domain at the C-terminus. Upon activation by its cytokine ligand, stem cell factor (SCF), this protein phosphorylates multiple intracellular proteins that play a role in in the proliferation, differentiation, migration and apoptosis of many cell types and thereby plays an important role in hematopoiesis, stem cell maintenance, gametogenesis, melanogenesis, and in mast cell development, migration and function. This protein can be a membrane-bound or soluble protein. Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/d3c3dcbf2b360d9c18c3e003d69b89ec3750f1857f1cd894194893227c568109.jpg?v=1666705917)
![Figure 2:Western blot analysis using KIT mAb against human KIT (AA: 23-322) recombinant protein. (Expected MW is 59.6 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/faddab5f8acf0b6a65b9d9126136a1b8a23a8af9c5f612a01514feb4aa331204.jpg?v=1666705917)
![Figure 3:Western blot analysis using KIT mAb against HEK293-6e (1) and KIT (AA: 23-322)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/3bc06ee55e6f1fe4590c51c8969a0d7debf59006fae9ee1ba4603fe4d4d129ce.jpg?v=1666705917)
![Figure 4:Immunofluorescence analysis of Hela cells using KIT mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/5e51214f7ea4767b5c4d25e235134d856fde0701e191bf924b9dce6dc62829e1.jpg?v=1666705917)
![Figure 5:Flow cytometric analysis of Hela cells using KIT mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ad9f8fa7b227cd263ed6041a23a2878d3ff2800b2a1fa5d8700e6865e9603167.jpg?v=1666705917)