KDM3A Primary Antibody

Item Information

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Price

Description

This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. JMJD1A functions as a mono- and dimethylation-specific demethylase, binding iron and alpha-ketoglutarate as cofactors and demethylating Lysine 9 of Histone H3. This suggests that JMJD1A plays a central role in the histone code and participates in nuclear hormone receptor-based transcriptional regulation. In addition, JMJD1A plays an important role in the regulation of cell growth during development and in chromatin regulation. JMJD1A directly regulates the expression of TNP1 and Protamine 1 (proteins required for the proper packaging and condensation of sperm chromatin) and, therefore, plays an essential role in spermatogenesis.

Product Overview

Entrez GenelD

55818

Aliases

TSGA; JMJD1; JHDM2A; JHMD2A; JMJD1A; KIAA0742; DKFZp686A24246; DKFZp686P07111; KDM3A

Clone#

2E1

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human KDM3A expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

ELISA

1/10000

References

1. DNA Res. 1998 Oct 30;5(5):277-86.
2. Proc Natl Acad Sci U S A.2004 Aug 17;101(33):12130-5.
3. Nature. 2005 Apr 7;434(7034):724-31.

Product Image

Western Blot

Figure 1: Western blot analysis using KDM3A mouse mAb against Hela (1) and HepG2 (2) cell lysate.

Immunofluorescence analysis

Figure 2: Immunofluorescence analysis of Hela cells using KDM3A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

For Research Use Only. Not for use in diagnostic procedures.