Mouse Monoclonal Antibody to KCNK1
Item Information
Catalog #
Size
Price
Description
This gene encodes one of the members of the superfamily of potassium channel proteins containing two pore-forming P domains. The product of this gene has not been shown to be a functional channel, however, it may require other non-pore-forming proteins for activity. [provided by RefSeq, Jul 2008]
Product Overview
Entrez GenelD
3775
Aliases
DPK; HOHO; K2P1; KCNO1; TWIK1; K2p1.1; TWIK-1
Clone#
3C1B8
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human KCNK1 (AA: extra mix) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Biophys J. 2016 Aug 23;111(4):775-784. 2,J Biol Chem. 2020 Jan 10;295(2):610-618.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/98db702039d90a0a40cb394aa39fb23aecdeb0021e863391cf436ddacb44aa08.jpg?v=1666705343)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using KCNK1 mAb against human KCNK1 (AA: extra mix) recombinant protein. (Expected MW is 36.7 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/3a75749c25abd334a3072f9fbb3e9fd9dc2f8bce9ed3504795aea856d88f16b0.jpg?v=1666705343)
Figure 2:Western blot analysis using KCNK1 mAb against human KCNK1 (AA: extra mix) recombinant protein. (Expected MW is 36.7 kDa)
Western Blot
![Figure 3:Western blot analysis using KCNK1 mAb against HEK293-6e (1) and KCNK1 (AA: extra mix)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/884a0469183deae4650a749d5841a09f5283b9b088f2f94c8d60d2c048e54a4e.jpg?v=1666705343)
Figure 3:Western blot analysis using KCNK1 mAb against HEK293-6e (1) and KCNK1 (AA: extra mix)-hIgGFc transfected HEK293-6e (2) cell lysate.
Flow cytometric analysis
![Figure 4:Flow cytometric analysis of C6 cells using KCNK1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/0b30534b566790de5e5217376792aded9fcffb3bea105ce982fd63190b85cdb5.jpg?v=1666705343)
Figure 4:Flow cytometric analysis of C6 cells using KCNK1 mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of HepG2 cells using KCNK1 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/55bb9bd91e25caa34915593a44c4bac6277f4bbda2722b2e09c824afd225f80c.jpg?v=1666705343)
Figure 5:Flow cytometric analysis of HepG2 cells using KCNK1 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using KCNK1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e291ba4771b74dbb95d2c029158c077787add15bec1db43e03f834946fe4ed9f.jpg?v=1666705343)
Figure 6:Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using KCNK1 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 7:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using KCNK1 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/35f6cb7493a78a772fe1e46f5d485235b5d3ba991a141fba85042f93bb8970fd.jpg?v=1666705343)
Figure 7:Immunohistochemical analysis of paraffin-embedded stomach cancer tissues using KCNK1 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.