IL3RA Primary Antibody

Item Information

Catalog #

Size

Price

Description

The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found.

Product Overview

Entrez GenelD

3563

Aliases

IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra

Clone#

10B8E7

Host / Isotype

Mouse / IgG1

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human IL3RA (AA: 200-305) expressed in E. Coli.

Formulation

Ascitic fluid containing 0.03% sodium azide.

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1. Am J Clin Pathol. 2011 Oct;136(4):625-30.
2. Chin Med J (Engl). 2010 Aug 5;123(15):2034-7.

Product Image

Western Blot

Figure 1: Western blot analysis using IL3RA mAb against human IL3RA recombinant protein. (Expected MW is 38.3 kDa)

Western Blot

Figure 2: Western blot analysis using IL3RA mAb against HEK293 (1) and IL3RA (AA: 200-305)-hIgGFc transfected HEK293 (2) cell lysate.

Immunofluorescence analysis

Figure 3: Immunofluorescence analysis of Hela cells using IL3RA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Flow cytometric