Mouse Monoclonal Antibody to IGF1R
Item Information
Catalog #
Size
Price
Description
This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Product Overview
Entrez GenelD
3480
Aliases
IGFR; CD221; IGFIR; JTK13
Clone#
3B4E3
Host / Isotype
Mouse / IgG2a
Immunogen
Purified recombinant fragment of human IGF1R (AA: extra(741-935)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Cells. 2019 Dec 14;8(12):1638.2,Nature. 2020 Jul;583(7817):615-619.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/fa2783bc0560a4a1db58c24404fa7dacf893c44b7e1d9d0cb13582113a3ef91f.jpg?v=1666705402)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using IGF1R mAb against human IGF1R (AA: extra(741-935)) recombinant protein. (Expected MW is 25.2 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/f6de5be29470a2921a4896fd2e1ea9368a4ff9ec6f7e3e65ffd5b478f8e7f519.jpg?v=1666705402)
Figure 2:Western blot analysis using IGF1R mAb against human IGF1R (AA: extra(741-935)) recombinant protein. (Expected MW is 25.2 kDa)
Western Blot
![Figure 3:Western blot analysis using IGF1R mAb against HEK293-6e (1) and IGF1R (AA: extra(741-935))-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/2469c290351465a204451143c5918e26c5a60bbf834371b42188a8ea344a88d8.jpg?v=1666705402)
Figure 3:Western blot analysis using IGF1R mAb against HEK293-6e (1) and IGF1R (AA: extra(741-935))-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
![Figure 4:Western blot analysis using IGF1R mouse mAb against NIH3T3 (1), cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/5bdf61fa90e8cc1966ec46b5b0e4b041d46a67cb8b768e1605a6b11f254c6b45.jpg?v=1666705402)
Figure 4:Western blot analysis using IGF1R mouse mAb against NIH3T3 (1), cell lysate.
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of HepG2 cells using IGF1R mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/1366b79a2b09914759832ae57582ac4b2abb12921913f1a43eaccbda825fc6f4.jpg?v=1666705402)
Figure 5:Flow cytometric analysis of HepG2 cells using IGF1R mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 6:Flow cytometric analysis of Lovo cells using IGF1R mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/56811302ac0395f935b46a83180276097a81dbab7732fe89f2c6b95c88f6c91b.jpg?v=1666705402)
Figure 6:Flow cytometric analysis of Lovo cells using IGF1R mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.