HLA-DRA Primary Antibody

Item Information

Catalog #

Size

Price

Description

HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.

Product Overview

Entrez GenelD

3122

Aliases

MLRW; HLA-DRA1

Clone#

1C11A5

Host / Isotype

Mouse / IgG2b

Species Reactivity

Human

Immunogen

Purified recombinant fragment of human HLA-DRA (AA: 26-254) expressed in E. Coli.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4°C; -20°C for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

ICC (Immunocytochemistry)

1/100 - 1/500

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Int J Immunogenet. 2014 Dec;41(6):508-11.
2.Am J Rhinol Allergy. 2012 Jan-Feb;26(1):12-7.

Product Image

Elisa

Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using HLA-DRA mAb against human HLA-DRA (AA: 26-254) recombinant protein. (Expected MW is 51.5 kDa)

Western Blot

Figure 3:Western blot analysis using HLA-DRA mAb against HEK293 (1) and HLA-DRA (AA: 26-254)-hIgGFc transfected HEK293 (2) cell lysate.

Western Blot

Figure 4:Western blot analysis using HLA-DRA mouse mAb against Ramos (1), and Raji (2) cell lysate.

Immunofluorescence analysis

Figure 5:Immunofluorescence analysis of Hela cells using HLA-DRA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric