FOXP3 Primary Antibody
Item Information
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Size
Price
Description
The protein encoded by this gene is a member of the forkhead/winged-helix family of transcriptional regulators. Defects in this gene are the cause of immunodeficiency polyendocrinopathy, enteropathy, X-linked syndrome (IPEX), also known as X-linked autoimmunity-immunodeficiency syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.
Product Overview
Entrez GenelD
50943
Aliases
JM2; AIID; IPEX; PIDX; XPID; DIETER
Clone#
4F12A2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human FOXP3 (AA: 297-431) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Mol Cancer. 2014 Jun 18;13:153.
2.Eur J Cancer. 2014 May;50(7):1291-300.
2.Eur J Cancer. 2014 May;50(7):1291-300.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
Western Blot
Figure 2:Western blot analysis using FOXP3 mAb against human FOXP3 (AA: 297-431) recombinant protein. (Expected MW is 42 kDa)
Western Blot
Figure 3:Western blot analysis using FOXP3 mAb against HEK293 (1) and FOXP3 (AA: 297-431)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of HeLa cells using FOXP3 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded liver cancer tissues using FOXP3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using FOXP3 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using FOXP3 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.