EIF5 Primary Antibody
Item Information
Catalog #
Size
Price
Description
Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).
Product Overview
Entrez GenelD
1983
Aliases
EIF-5; EIF-5A
Clone#
6D6G6
Host / Isotype
Mouse / IgG2a
Species Reactivity
Human, Monkey
Immunogen
Purified recombinant fragment of human EIF5 (AA: 1-300) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ELISA
1/10000
References
1.Biochemistry. 2006 Apr 11;45(14):4550-8.
2.Biochem Biophys Res Commun. 2011 Dec 2;415(4):567-72.
2.Biochem Biophys Res Commun. 2011 Dec 2;415(4):567-72.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using EIF5 mAb against human EIF5 (AA: 1-300) recombinant protein. (Expected MW is 60.2 kDa)
Western Blot
Figure 3:Western blot analysis using EIF5 mAb against HEK293 (1) and EIF5 (AA: 1-300)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using EIF5 mouse mAb against K562 (1), COS7 (2), Hela (3), and A431 (4) cell lysate.
For Research Use Only. Not for use in diagnostic procedures.