EGFR mutant Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
Product Overview
Entrez GenelD
1956
Aliases
ERBB; HER1; mENA; ERBB1; PIG61
Clone#
5G9B5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human EGFR mutant (AA: 693-893) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1. J Immunol. 2012 Dec 1;189(11):5230-9.
2. J Biol Chem. 2012 Oct 12;287(42):35201-11.
2. J Biol Chem. 2012 Oct 12;287(42):35201-11.
Product Image
Western Blot
Figure 1: Western blot analysis using EGFR mutant mAb against human EGFR mutant recombinant protein. (Expected MW is 36.9 kDa)
Western Blot
Figure 2: Western blot analysis using EGFR mutant mAb against HEK293 (1) and EGFR mutant (AA: 693-893)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 3: Western blot analysis using EGFR mutant mouse mAb against SPC-A-1 (1), A549 (2), HepG2 (3) and MCF-7 (4) cell lysate.
Flow cytometric
Figure 4: Flow cytometric analysis of HepG2 cells using EGFR mutant mouse mAb (green) and negative control (purple).
Immunohistochemical analysis
Figure 5: Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using EGFR mutant mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using EGFR mutant mouse mAb with DAB staining.
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.