Mouse Monoclonal Antibody to CEACAM6
Item Information
Catalog #
Size
Price
Description
This gene encodes a protein that belongs to the carcinoembryonic antigen (CEA) family whose members are glycosyl phosphatidyl inositol (GPI) anchored cell surface glycoproteins. Members of this family play a role in cell adhesion and are widely used as tumor markers in serum immunoassay determinations of carcinoma. This gene affects the sensitivity of tumor cells to adenovirus infection. The protein encoded by this gene acts as a receptor for adherent-invasive E. coli adhesion to the surface of ileal epithelial cells in patients with Crohn's disease. This gene is clustered with genes and pseudogenes of the cell adhesion molecules subgroup of the CEA family on chromosome 19.
Product Overview
Entrez GenelD
4680
Aliases
NCA; CEAL; CD66c
Clone#
4B9F1
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human CEACAM6 (AA: 35-256) expressed in HEK293-6e cells supernatant.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Sci Rep. 2019 Dec 4;9(1):18347. 2.J Immunol. 2017 Apr 15;198(8):3307-3317.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/2ebe4ad5fa13fe6d7584357f663ae7fa9f2dc0219499ce203ac898648f586411.jpg?v=1666705712)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using CEACAM6 mAb against human CEACAM6 (AA: 35-256) recombinant protein. (Expected MW is 55 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/4be6384aa4e457362194b402ebd3e8a816fafd777795bc7e2055bed7dd4e6b9f.jpg?v=1666705712)
Figure 2:Western blot analysis using CEACAM6 mAb against human CEACAM6 (AA: 35-256) recombinant protein. (Expected MW is 55 kDa)
Immunofluorescence analysis
![Figure 3:Immunofluorescence analysis of Hela cells using CEACAM6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/02ab40af153b55e8f6ddec4b36c6b428c4f543228021500ee46a2764152e72b4.jpg?v=1666705713)
Figure 3:Immunofluorescence analysis of Hela cells using CEACAM6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric analysis
![Figure 4:Flow cytometric analysis of Jurkat cells using CEACAM6 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/e0256397c6192b0ed79cdcdaa6de6ee52411f1e9723d0fcecea0ff37fd257c9e.jpg?v=1666705713)
Figure 4:Flow cytometric analysis of Jurkat cells using CEACAM6 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
![Figure 5:Immunohistochemical analysis of paraffin-embedded esophagus tissues using CEACAM6 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ff8e902a32359adb19c83c473b82671c8ed68584684dba4419776a6c3c7e2521.jpg?v=1666705713)
Figure 5:Immunohistochemical analysis of paraffin-embedded esophagus tissues using CEACAM6 mouse mAb with DAB staining.
Immunohistochemical analysis
![Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CEACAM6 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/62885c2bf88f4e7abebeb0e72b4a88560d888fd798ae90abc6b066a5d2b99ff2.jpg?v=1666705713)
Figure 6:Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CEACAM6 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.