Mouse Monoclonal Antibody to CEACAM6

Item Information

Catalog #

Size

Price

Description

This gene encodes a protein that belongs to the carcinoembryonic antigen (CEA) family whose members are glycosyl phosphatidyl inositol (GPI) anchored cell surface glycoproteins. Members of this family play a role in cell adhesion and are widely used as tumor markers in serum immunoassay determinations of carcinoma. This gene affects the sensitivity of tumor cells to adenovirus infection. The protein encoded by this gene acts as a receptor for adherent-invasive E. coli adhesion to the surface of ileal epithelial cells in patients with Crohn's disease. This gene is clustered with genes and pseudogenes of the cell adhesion molecules subgroup of the CEA family on chromosome 19.

Product Overview

Entrez GenelD

4680

Aliases

NCA; CEAL; CD66c

Clone#

4B9F1

Host / Isotype

Mouse / IgG1

Immunogen

Purified recombinant fragment of human CEACAM6 (AA: 35-256) expressed in HEK293-6e cells supernatant.

Formulation

Purified antibody in PBS with 0.05% sodium azide

Storage

4℃; -20℃ for long term storage

Product Applications

WB (Western Blot)

1/500 - 1/2000

IHC_P(Immunohistochemistry)

1/200 - 1/1000

ICC (Immunocytochemistry)

1/200 - 1/1000

FCM (Flow Cytometry)

1/200 - 1/400

ELISA

1/10000

References

1.Sci Rep. 2019 Dec 4;9(1):18347. 2.J Immunol. 2017 Apr 15;198(8):3307-3317.

Product Image

Elisa

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Western Blot

Figure 2:Western blot analysis using CEACAM6 mAb against human CEACAM6 (AA: 35-256) recombinant protein. (Expected MW is 55 kDa)

Immunofluorescence analysis

Figure 3:Immunofluorescence analysis of Hela cells using CEACAM6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Flow cytometric analysis