CD82
Item Information
Catalog #
Size
Price
Description
This metastasis suppressor gene product is a membrane glycoprotein that is a member of the transmembrane 4 superfamily. Expression of this gene has been shown to be downregulated in tumor progression of human cancers and can be activated by p53 through a consensus binding sequence in the promoter. Its expression and that of p53 are strongly correlated, and the loss of expression of these two proteins is associated with poor survival for prostate cancer patients. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Product Overview
Entrez GenelD
3732
Aliases
R2; 4F9; C33; IA4; ST6; GR15; KAI1; SAR2; TSPAN27
Clone#
5D5D5
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human CD82 (AA: extra 111-228) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Cells. 2021 Jun 18;10(6):1545.
2.Sci Rep. 2021 May 5;11(1):9530.
2.Sci Rep. 2021 May 5;11(1):9530.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD82 mAb against human CD82 (AA: extra 111-228) recombinant protein. (Expected MW is 39.5 kDa)
Western Blot
Figure 3:Western blot analysis using CD82 mAb against HEK293-6e (1) and CD82 (AA: extra 111-228)-hIgGFc transfected HEK293-6e (2) cell lysate.
Immunofluorescence analysis
Figure 4:Flow cytometric analysis of Raji cells using CD82 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 5:Immunohistochemical analysis of paraffin-embedded human tonsil tissues using CD82 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 6:Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using CD82 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded rabbit stomach tissues using CD82 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded rabbit small intestine tissues using CD82 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.