Mouse Monoclonal Antibody to CD319

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Description

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. Isoform 1 mediates NK cell activation through a SH2D1A-independent extracellular signal-regulated ERK-mediated pathway (PubMed:11698418). Positively regulates NK cell functions by a mechanism dependent on phosphorylated SH2D1B. Downstream signaling implicates PLCG1, PLCG2 and PI3K (PubMed:16339536). In addition to heterotypic NK cells-target cells interactions also homotypic interactions between NK cells may contribute to activation. However, in the absence of SH2D1B, inhibits NK cell function. Acts also inhibitory in T-cells (By similarity). May play a role in lymphocyte adhesion (PubMed:11802771). In LPS-activated monocytes negatively regulates production of proinflammatory cytokines (PubMed:23695528).

Product Overview
Entrez GenelD
57823
Aliases
19A; CS1; SLAMF7; CRACC
Clone#
7A8D5
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human CD319 (AA: extra(23-226)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Leuk Lymphoma. 2019 Dec;60(13):3335-3338. 2,J Immunol. 2019 Jan 1;202(1):228-238.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD319 mAb against human CD319 (AA: extra(23-226)) recombinant protein. (Expected MW is 48.3kDa)
Figure 2:Western blot analysis using CD319 mAb against human CD319 (AA: extra(23-226)) recombinant protein. (Expected MW is 48.3kDa)
Western Blot
Figure 3:Western blot analysis using CD319 mAb against HEK293-6e (1) and CD319 (AA: extra(23-226))-hIgGFc transfected HEK293-6e (2) cell lysate.
Figure 3:Western blot analysis using CD319 mAb against HEK293-6e (1) and CD319 (AA: extra(23-226))-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CD319 mouse mAb against K562 (1), HL-60 (2), Ramos (3), Jurkat (4), and THP-1 (5) cell lysate.
Figure 4:Western blot analysis using CD319 mouse mAb against K562 (1), HL-60 (2), Ramos (3), Jurkat (4), and THP-1 (5) cell lysate.
Flow cytometric analysis
Figure 5:Flow cytometric analysis of K562 cells using CD319 mouse mAb (green) and negative control (red).
Figure 5:Flow cytometric analysis of K562 cells using CD319 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 6:Flow cytometric analysis of U937 cells using CD319 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of U937 cells using CD319 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 7:Flow cytometric analysis of THP-1 cells using CD319 mouse mAb (green) and negative control (red).
Figure 7:Flow cytometric analysis of THP-1 cells using CD319 mouse mAb (green) and negative control (red).
Flow cytometric analysis
Figure 8:Flow cytometric analysis of MOLT4 cells using CD319 mouse mAb (green) and negative control (red).
Figure 8:Flow cytometric analysis of MOLT4 cells using CD319 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 9:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using CD319 mouse mAb with DAB staining.
Figure 9:Immunohistochemical analysis of paraffin-embedded breast cancer tissues using CD319 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 10:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using CD319 mouse mAb with DAB staining.
Figure 10:Immunohistochemical analysis of paraffin-embedded rectal cancer tissues using CD319 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.

Catalog#: 32123

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