Mouse Monoclonal Antibody to CD273
Item Information
Catalog #
Size
Price
Description
PDCD1LG2 (Programmed Cell Death 1 Ligand 2) is a Protein Coding gene. Diseases associated with PDCD1LG2 include Cysticercosis and Testicular Lymphoma. Among its related pathways are Innate Immune System and Class I MHC mediated antigen processing and presentation. An important paralog of this gene is CD274.
Product Overview
Entrez GenelD
80380
Aliases
B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
Clone#
5F4H10
Host / Isotype
Mouse / IgG2b
Immunogen
Purified recombinant fragment of human CD273 (AA: 20-220) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,J Immunother Cancer. 2019 Aug 29;7(1):233. 2,Cancer Sci. 2020 Apr;111(4):1113-1123.
Product Image
Elisa
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/bdf9a7892d2a7846f142ebff5b256ba137dea6fe54b39cc04becd9ac6eb0ff2c.jpg?v=1666705316)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
![Figure 2:Western blot analysis using CD273 mAb against human CD273 (AA: 20-220) recombinant protein. (Expected MW is 48.6 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/3d1e724b31aa709bed3ff41e198a0216a4f26b8f0790d2fa4cbdaffbc7a18854.jpg?v=1666705316)
Figure 2:Western blot analysis using CD273 mAb against human CD273 (AA: 20-220) recombinant protein. (Expected MW is 48.6 kDa)
Western Blot
![Figure 3:Western blot analysis using CD273 mouse mAb against HepG2 (1), Hela (2),NIH/3T3 (3),PC-12 (4),K562 (5),Jurkat (6),MCF-7 (7), and HEK293-6e (8) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/5285438972e0423311b667cf54e0a285eda2d7e4c9d11a501a046ecdc4d2dcc5.jpg?v=1666705316)
Figure 3:Western blot analysis using CD273 mouse mAb against HepG2 (1), Hela (2),NIH/3T3 (3),PC-12 (4),K562 (5),Jurkat (6),MCF-7 (7), and HEK293-6e (8) cell lysate.
Flow cytometric analysis
![Figure 4:Flow cytometric analysis of Hela cells using CD273 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/ae2bf928bf9588cb47c3864aeb77e02964d7a50a49b429b44f7a1ecad953a0b7.jpg?v=1666705316)
Figure 4:Flow cytometric analysis of Hela cells using CD273 mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 5:Flow cytometric analysis of Jurkat cells using CD273 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/3ea07d84e8f0246505147936f2352388df34d45b5c20747aa6ed3f9b51a89a86.jpg?v=1666705316)
Figure 5:Flow cytometric analysis of Jurkat cells using CD273 mouse mAb (green) and negative control (red).
Flow cytometric analysis
![Figure 6:Flow cytometric analysis of K562 cells using CD273 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/fe8c61586f8dfe811a7573e96bd1145c8d299b2cc71f9f31c6c0c8d4c37a7013.jpg?v=1666705316)
Figure 6:Flow cytometric analysis of K562 cells using CD273 mouse mAb (green) and negative control (red).
Immunofluorescence analysis
![Figure 7:Immunofluorescence analysis of Hela cells using CD273 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/88173235c404130fefdecbfe946ce1fab30c7a9b8dfe809ebbf4c843209d6393.jpg?v=1666705316)
Figure 7:Immunofluorescence analysis of Hela cells using CD273 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.