CD137 Primary Antibody
Item Information
Catalog #
Size
Price
Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB.
Product Overview
Entrez GenelD
3604
Aliases
TNFRSF9; ILA; 4-1BB; CDw137
Clone#
2C6B4
Host / Isotype
Mouse / Mouse IgG1
Immunogen
Purified recombinant fragment of human CD137 (AA: 214-255) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200-1/400
ELISA
1/10000
References
1.Blood. 2019 Oct 31;134(18):1510-1516. 2.Cancer Commun (Lond). 2019 Jul 9;39(1):41.
Product Image
ELISA
![Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-elisa_31912ea_353.jpg?v=1666704890)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng)
WESTERN BLOT
![Figure 2: Western blot analysis using CD137 mAb against human CD137 (AA: 214-255) recombinant protein. (Expected MW is 30.9 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-wb_31912rwb_354.jpg?v=1666704890)
Figure 2: Western blot analysis using CD137 mAb against human CD137 (AA: 214-255) recombinant protein. (Expected MW is 30.9 kDa)
WESTERN BLOT
![Figure 3: Western blot analysis using CD137 mAb against HEK293-6e (1) and CD137 (AA: 214-255)-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-wb_31912twb_355.jpg?v=1666704890)
Figure 3: Western blot analysis using CD137 mAb against HEK293-6e (1) and CD137 (AA: 214-255)-hIgGFc transfected HEK293-6e (2) cell lysate.
WESTERN BLOT
![Figure 4: Western blot analysis using CD137 mouse mAb against HepG2 (1), Jurkat (2), MOLT4 (3), HL-60 (4), THP-1 (5), Ramos (6), K562 (7), and Raji (8) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-wb_31912lwb_356.jpg?v=1666704890)
Figure 4: Western blot analysis using CD137 mouse mAb against HepG2 (1), Jurkat (2), MOLT4 (3), HL-60 (4), THP-1 (5), Ramos (6), K562 (7), and Raji (8) cell lysate.
FLOW CYTOMETRY
![Figure 5: Flow cytometric analysis of HL-60 cells using CD137 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-fcm_31912fcm-1_357.jpg?v=1666704890)
Figure 5: Flow cytometric analysis of HL-60 cells using CD137 mouse mAb (green) and negative control (red).
FLOW CYTOMETRY
![Figure 6: Flow cytometric analysis of THP-1 cells using CD137 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/graph-fcm_31912fcm-2_358.jpg?v=1666704890)
Figure 6: Flow cytometric analysis of THP-1 cells using CD137 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.