CD130 Primary Antibody

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Description

The protein encoded by this gene is a signal transducer shared by many cytokines, including interleukin 6 (IL6), ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF), and oncostatin M (OSM). This protein functions as a part of the cytokine receptor complex. The activation of this protein is dependent upon the binding of cytokines to their receptors. vIL6, a protein related to IL6 and encoded by the Kaposi sarcoma-associated herpesvirus, can bypass the interleukin 6 receptor (IL6R) and directly activate this protein. Knockout studies in mice suggest that this gene plays a critical role in regulating myocyte apoptosis. Alternatively spliced transcript variants have been described. A related pseudogene has been identified on chromosome 17. [provided by RefSeq, May 2014]

Product Overview
Entrez GenelD
3572
Aliases
IL6ST; GP130; CDW130; IL-6RB
Clone#
8D4C5
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Monkey
Immunogen
Purified recombinant fragment of human CD130 (AA: extra 73-231) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.J Exp Med. 2017 Sep 4;214(9):2547-2562.
2.Biochem Biophys Res Commun. 2016 Feb 19;470(4):870-6.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD130 mAb against human CD130 (AA: extra 73-231) recombinant protein. (Expected MW is 43.8 kDa)
Figure 2:Western blot analysis using CD130 mAb against human CD130 (AA: extra 73-231) recombinant protein. (Expected MW is 43.8 kDa)
Western Blot
Figure 3:Western blot analysis using CD130 mAb against HEK293 (1) and CD130 (AA: extra 73-231)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using CD130 mAb against HEK293 (1) and CD130 (AA: extra 73-231)-hIgGFc transfected HEK293 (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using CD130 mouse mAb against COS7 (1) cell lysate.
Figure 4:Western blot analysis using CD130 mouse mAb against COS7 (1) cell lysate.
Immunofluorescence analysis
Figure 5:Immunofluorescence analysis of Hela cells using CD130 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 5:Immunofluorescence analysis of Hela cells using CD130 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Flow cytometric
Figure 6:Flow cytometric analysis of HL-60 cells using CD130 mouse mAb (green) and negative control (red).
Figure 6:Flow cytometric analysis of HL-60 cells using CD130 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.

Catalog#: 31686

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