Mouse Monoclonal Antibody to CD121B
Item Information
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Description
The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. Interleukin 4 (IL4) is reported to antagonize the activity of interleukin 1 by inducing the expression and release of this cytokine. This gene and three other genes form a cytokine receptor gene cluster on chromosome 2q12. Alternative splicing results in multiple transcript variants and protein isoforms. Alternative splicing produces both membrane-bound and soluble proteins. A soluble protein is also produced by proteolytic cleavage.
Product Overview
Entrez GenelD
7850
Aliases
IL1R2;IL1RB; IL1R2c; CDw121b; IL-1R-2; IL-1RT2; IL-1RT-2
Clone#
3G1D8
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human CD121B (AA: extra 14-343) expressed in HEK293-6e cells supernatant.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Mol Genet Genomic Med. 2019 May;7(5):e644. 2.Mol Genet Genomic Med. 2019 Jan;7(1):e00516.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using CD121B mAb against human CD121B (AA: extra 14-343) recombinant protein. (Expected MW is 68.3 kDa)
Western Blot
Figure 3:Western blot analysis using CD121B mouse mAb against Hela (1) and MCF-7 (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of THP-1 cells using CD121B mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.