Mouse Monoclonal Antibody to CD10
This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing.
![Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/d17378810bdffeaa3b7b75ec991a4044253a4b0285e5681082f8d323bb07c3b9.jpg?v=1666705463)
![Figure 2:Western blot analysis using CD10 mAb against human CD10 (AA: (651-750)) recombinant protein. (Expected MW is 38.2 kDa)](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/a2f745a051dbd066c4843035697f81c8d710c0cb531d9598284a7851c479d052.jpg?v=1666705464)
![Figure 3:Western blot analysis using CD10 mAb against HEK293-6e (1) and CD10 (AA: (651-750))-hIgGFc transfected HEK293-6e (2) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/fc474fec882dc03fadcb9be45f3adef82e908c9cd1669b04e06f9ae01e06a23c.jpg?v=1666705464)
![Figure 4:Western blot analysis using CD10 mouse mAb against Raji (1), Ramos (2), and LNcap (3) cell lysate.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/942e39fa1696585b7a76232b3f12b4b6938706b9606fb7261f26e1a370363f72.jpg?v=1666705464)
![Figure 5:Flow cytometric analysis of Hela cells using CD10 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/96f34ad5a399250b10018ba29421d8a8cec76eb99045de731b258eca3b264752.jpg?v=1666705464)
![Figure 6:Flow cytometric analysis of THP-1 cells using CD10 mouse mAb (green) and negative control (red).](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/cf6675a8e35196a8afe6e422583a3bb322806a9e7082ba4b5bc6727b28d065f6.jpg?v=1666705464)
![Figure 7:Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CD10 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/fb0df2822c9c4a2a7f989645b05b5991c915f342bd386b0bd006cfc3c1cb7dc0.jpg?v=1666705464)
![Figure 8:Immunohistochemical analysis of paraffin-embedded brain tissues using CD10 mouse mAb with DAB staining.](https://cdn.shopify.com/s/files/1/0604/2661/7019/products/cef35655e8388e7cb64def17e95cb56cf76b9c8a2748ece670f44326213aa857.jpg?v=1666705464)