BCL6
Item Information
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Size
Price
Description
The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene.
Product Overview
Entrez GenelD
604
Aliases
BCL5; LAZ3; BCL6A; ZNF51; ZBTB27
Clone#
7A6D1
Host / Isotype
Mouse / Mouse IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human BCL6 (AA: 507-706) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
IHC_P(Immunohistochemistry)
1/200 - 1/1000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Blood. 2021 Feb 11;137(6):812-825.
2.Int J Mol Sci. 2020 Nov 9;21(21):8393.
2.Int J Mol Sci. 2020 Nov 9;21(21):8393.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using BCL6 mAb against human BCL6 (AA: 507-706) recombinant protein. (Expected MW is 26 kDa)
Western Blot
Figure 3:Western blot analysis using BCL6 mAb against HEK293-6e (1) and BCL6 (AA: 507-706)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using BCL6 mouse mAb against Jurkat (1), K562 (2), Raji (3), Ramos (4) and MOLT-4 (5) cell lysate.
Immunohistochemical analysis
Figure 5:Immunofluorescence analysis of Hela cells using BCL6 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Immunofluorescence analysis
Figure 6:Flow cytometric analysis of HL-60 cells using BCL6 mouse mAb (green) and negative control (red).
Immunohistochemical analysis
Figure 7:Immunohistochemical analysis of paraffin-embedded lung cancer tissues using BCL6 mouse mAb with DAB staining.
Immunohistochemical analysis
Figure 8:Immunohistochemical analysis of paraffin-embedded cervical carcinoma tissues using BCL6 mouse mAb with DAB staining.
For Research Use Only. Not for use in diagnostic procedures.