Mouse Monoclonal Antibody to ATP6AP1
Item Information
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Description
This gene encodes a component of a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules. This protein may also play a role in early development.
Product Overview
Entrez GenelD
537
Aliases
16A; CF2; Ac45; XAP3; XAP-3; ATP6S1; VATPS1; ATP6IP1
Clone#
3H11B12
Host / Isotype
Mouse / IgG1
Immunogen
Purified recombinant fragment of human ATP6AP1 (AA: 51-151) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4℃; -20℃ for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1,Histol Histopathol. 2007 Apr;22(4):443-54. 2,Eur J Med Genet. 2020 Jun;63(6):103882.
Product Image
Elisa
Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ATP6AP1 mAb against human ATP6AP1 (AA: 51-151) recombinant protein. (Expected MW is 37kDa)
Western Blot
Figure 3:Western blot analysis using ATP6AP1 mAb against HEK293-6e (1) and human ATP6AP1 (AA: 51-151)-hIgGFc transfected HEK293-6e (2) cell lysate.
Western Blot
Figure 4:Western blot analysis using ATP6AP1 mouse mAb against PC-3 (1), and Raw264.7 (2) cell lysate.
Flow cytometric analysis
Figure 4:Flow cytometric analysis of Hela cells using ATP6AP1 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.