ATG2A Primary Antibody

Item Information
Catalog #
Size
Price
Description
ATG2A (Autophagy Related 2A) is a Protein Coding gene. An important paralog of this gene is ATG2B.
Product Overview
Entrez GenelD
23130
Aliases
N
Clone#
4H8G3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human ATG2A (AA: 325-429) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Mol Biol Cell. 2012 Mar;23(5):896-909.
2.Acta Biochim Pol. 2011;58(3):365-74.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using ATG2A mAb against human ATG2A (AA: 325-429) recombinant protein. (Expected MW is 37.2 kDa)
Figure 2:Western blot analysis using ATG2A mAb against human ATG2A (AA: 325-429) recombinant protein. (Expected MW is 37.2 kDa)
Western Blot
Figure 3:Western blot analysis using ATG2A mAb against HEK293 (1) and ATG2A (AA: 325-429)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 3:Western blot analysis using ATG2A mAb against HEK293 (1) and ATG2A (AA: 325-429)-hIgGFc transfected HEK293 (2) cell lysate.
Immunofluorescence analysis
Figure 4:Immunofluorescence analysis of K562 cells using ATG2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
Figure 4:Immunofluorescence analysis of K562 cells using ATG2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
For Research Use Only. Not for use in diagnostic procedures.