ARHGDIA Primary Antibody
Item Information
Catalog #
Size
Price
Description
Aplysia Ras-related homologs (ARHs), also called Rho genes, belong to the RAS gene superfamily encoding small guanine nucleotide exchange (GTP/GDP) factors. The ARH proteins may be kept in the inactive, GDP-bound state by interaction with GDP dissociation inhibitors, such as ARHGDIA
Product Overview
Entrez GenelD
396
Aliases
GDIA1;RHOGDI;RHOGDI-1
Clone#
2G3
Host / Isotype
Mouse / IgG1
Species Reactivity
Human, Mouse, Monkey
Immunogen
Purified recombinant fragment of human ARHGDIA (AA: FULL(1-204)) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
ICC (Immunocytochemistry)
1/200 - 1/1000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Nat Cell Biol. 2010 May;12(5):477-83.
2.Int J Oncol. 2010 Feb;36(2):379-86.
2.Int J Oncol. 2010 Feb;36(2):379-86.
Product Image
Western Blot
Figure 1: Western blot analysis using ARHGDIA mAb against human ARHGDIA recombinant protein. (Expected MW is 48.7 kDa)
Western Blot
Figure 2: Western blot analysis using ARHGDIA mouse mAb against Jurkat (1), HeLa (2), NIH3T3 (3), C6 (4), K562 (5), and COS7 (6) cell lysate.
Immunofluorescence analysis
Figure 3: Immunofluorescence analysis of HepG2 cells using ARHGDIA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow cytometric
Figure 4: Flow cytometric analysis of HeLa cells using ARHGDIA mouse mAb (green) and negative control (red).
Elisa
Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
For Research Use Only. Not for use in diagnostic procedures.