AKT3 Primary Antibody
Item Information
Catalog #
Size
Price
Description
This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.
Product Overview
Entrez GenelD
1000
Aliases
CDHN; NCAD; CD325; CDw325
Clone#
7B7A2
Host / Isotype
Mouse / IgG1
Species Reactivity
Human
Immunogen
Purified recombinant fragment of human AKT3 (AA: 37-150) expressed in E. Coli.
Formulation
Purified antibody in PBS with 0.05% sodium azide
Storage
4°C; -20°C for long term storage
Product Applications
WB (Western Blot)
1/500 - 1/2000
FCM (Flow Cytometry)
1/200 - 1/400
ELISA
1/10000
References
1.Mol Med Rep. 2015 Aug;12(2):2999-3006.
2.BMC Cancer. 2013 Jun 26;13:309.
2.BMC Cancer. 2013 Jun 26;13:309.
Product Image
Elisa
Figure 1: Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Western Blot
Figure 2:Western blot analysis using AKT3 mAb against human AKT3 (AA: 37-150) recombinant protein. (Expected MW is 39.5 kDa)
Western Blot
Figure 3:Western blot analysis using AKT3 mAb against HEK293 (1) and AKT3 (AA: 37-150)-hIgGFc transfected HEK293 (2) cell lysate.
Flow cytometric
Figure 4:Flow cytometric analysis of Hela cells using AKT3 mouse mAb (green) and negative control (red).
For Research Use Only. Not for use in diagnostic procedures.