CD22scFv-TM28-CD28-CD3z
Item Information
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Description
ProMab's PM-CAR1029 primary human CAR-T cells express an anti-CD22 CAR containing a CD22-specific scFv, hinge and transmembrane domains, the CD28 costimulatory domain and the CD3zeta activation domain.
Product Overview
Cell Type Tested
T cells
Formulation
CS10 CryoStor Medium (Sigma Aldrich, Cat, #C2874)
Cell Viability
>90%
Mycoplasma Testing
The cells has been screened using a PCR-based mycoplasma detection kit to conirm the absence of mycoplasma species.
Storage
-80°C; liquid nitrogen
Shipping & Handing
This product will be shipped in a box containing dry ice at a temperature of -70°C.
Product Image
CAR Structure
Figure 1. Schematic representation of the scFv, costimulatory domain, and activation domain of PM-CAR1029. This construct targets CD22.
Facs
Figure 2. FACS analysis with human FAB antibody shows expression of CD22-CAR.
RTCA
Figure 3. Top panel: RTCA cytotoxicity activity of effector CD22-CD28-CD3-CAR-T cells against CHO-CD22 (target cells available from ProMab). CD22-Beam2-CAR-T cells with tag after ScFv to detect CD22 are also shown. Effector: Target cells ratio=10:1. Bottom panel: RTCA cytotoxicity activity of effector CD22-CD28-CD3- CAR-T cells against parental CHO target cells (available from ProMab).
For Research Use Only. Not for use in diagnostic procedures.